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School Programme
Main Lecture Topics
- The Fourier transform in 1D - 2D - 3D
- Properties of the Fourier transform
- Specimen preparation for single-particle cryo-EM
- (Automatic) Particle selection
- Optimizing the EM data collection
- CTF correction
- Filtering and data normalization
- Alignment using correlation functions
- Multi-reference alignments
- Multivariate statistics and classification
- Angular reconstitution
- Three-dimensional reconstruction
- Projection matching
- Alignment refinements
- 3D refinements
- 3D resolution criteria
- 3D fitting of known X-ray structures
- Refining towards different 3D structures simultaneously ("4D" analysis)
- Using image processing software (IMAGIC, FREALIGN)
- Parallel processing
Participant's Talks
- Presenting the own project
Daily School Schedule
- Two or three morning lectures
- Hands-on single particle analysis
- Lunch
- Open afternoons / Time for additional workshops
- Evening lectures / Hands-on processing
- Dinner
- Open program after dinner
Leisure Programme
- Swimming Pool
- Tours and trips
- Rafting, horse riding, sports
- Churasco evenings
- Caipirinha night
- More...