Santa Rita School
for
Single Particle Cryo-EM

18-30 August 2005

 

Main Lecture topics

  • The Fourier Transform in 1D - 2D - 3D
  • Properties of the Fourier Transform
  • Specimen Preparation for single-particle cryo-EM
  • (Automatic) Particle Selection
  • Using the IMAGIC software system
  • Filtering and Data Normalization
  • Optimizing the EM data collection (CTF)
  • CTF correction
  • Alignment using Correlation Functions
  • Multi Reference Alignments
  • Multivariate Statistics and Classification
  • Three Dimensional Reconstruction
  • Angular Reconstitution
  • Projection Matching
  • Parallel processing on computer farms
  • Alignment Refinements
  • 3D Refinements
  • 3D Rendering
  • 3D Fitting known X-ray Structures
  • 3D resolution criteria
  • Refining towards different 3D structures simultaneously
  • Advanced topics (including Solvent Flattening)

 

 

Lecturers

  • Bruno Klaholz
  • Holger Stark
  • Michael Schatz
  • Marin van Heel
  • Ralf Schmidt
  • Igor Polikarpov
  • Elena Orlova
  • Ardan Patwardhan

     

 

 

 

 

 

Daily School Schedule

  • Two or three morning lectures (8:30 am - 10:30 am)
  • Hands on single particle analysis.
  • Lunch (1 pm - 2 pm)
  • Afternoons open (2 pm - 5 pm)
  • 5 pm - 8 pm: Evening lectures / Hands-on processing
  • Dinner 8 - 9 pm
  • Open program after dinner

Leisure Programme

  • White water rafting
  • Gloria Swimming Pool
  • Mountain biking
  • Horseback riding
  • Two Churasco Evenings
  • The waterfalls of Santa Rita
  • Canoeing on the Mogi Guass